max in binding experiments, respectively. Statistical analysis of person representative curves was done by multiple t
Significant genotype variations in the number of synapses are indicated in italics. Structured illumination microscopy of WT and KI midbrain slices was utilized to quantify the colocalization of your outlined proteins within SERT-beneficial synapses.
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max in WT synaptoneurosomes, suggesting that Src signaling can be downregulated in KI synapses. However, our capacity to rescue SERT functionality in KI midbrain synaptoneurosomes through the inhibition of FAK implies elevated FAK signaling downstream from the Pro32Pro33 mutant, as verified by amplified pFAK localization in 5-HT synapses. Our info therefore recommend the Src–FAK pathway is uncoupled by constitutive activation of integrin βthree, and thus Every single kinase may very well be influencing SERT independently.
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